• Smith-Root | Technology For Fisheries Conservation
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  • Company News
  • Posted Sunday, February 19, 2017

Field Kits for Environmental DNA and Fish Pathogen Detection Now Available!

Smith-Root and Biomeme are happy to announce the release of eleven species-specific assays developed for use with Biomeme’s two3™ handheld qPCR device. The eleven assays were developed with input from leading environmental DNA practitioners and fish health professionals. All assays were developed using peer-reviewed, published DNA sequences unique to the target species, and all have undergone rigorous validation testing with standard bench-top qPCR equipment.

Five of the newly developed assays are for detection of invasive or threatened and endangered species using filtered water samples. The remaining six assays—which can be used with a tissue, swab, or blood samples—are for detection of pathogens commonly found at aquaculture facilities.

Environmental DNA Tests

  • Sea lamprey (Petromyzon marinus)
  • Silver carp and Bighead carp (Hypophthalmichthys molitrix and H. nobilis)
  • New Zealand mudsnails (Potamopyrgus antipodarum)
  • Eastern hellbenders (Cryptobranchus alleganiensis)
  • Grass carp (Ctenopharyngodon idella)

Aquaculture Pathogen Tests

  • Bacterial cold water disease (Flavobacterium psychrophilum)
  • Tenacibaculosis (Tenacibaculum maritimum)
  • Columnaris (Flavobacterium columnare)
  • Amoebic gill disease (Neoparamoeba perurans)
  • Enteric septicemia of catfish (Edwarsiella ictualuri)
  • Bacterial kidney disease (Renibacterium salmoninarum)

After sample collection, the process of extracting DNA for detection using Biomeme’s process is relatively quick and easy. Field prep and DNA extraction generally takes ~5-7 minutes per test strip (3 samples/test strip), and results are provided in 45 minutes. Voila! From there, data is uploaded and stored in a cloud portal service.

For more information, please visit http://www.smith-root.com/edna/

About Smith-Root

Founded in 1964, Smith-Root has long been a respected member of the aquatic resource conservation community. We have supplied fish researchers, managers and hatchery personnel with quality fisheries sampling technologies and products over several decades. With on-site manufacturing facilities, Smith-Root produces a full-line of electrofishing, fish guidance, and electrosedation equipment to aid in fish restoration and recovery operations in various aquatic settings.