• Smith-Root | Technology For Fisheries Conservation
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eDNA Laboratory Services

Smith-Root is exclusively partnered with Precision Biomonitoring—a spin-off from the Biodiversity Institute at the University of Guelph (home of the Barcode of Life Project)—to offer our customers laboratory analysis for their environmental DNA (eDNA) samples. While we also offer rapid, onsite qPCR results through our Biomeme platform, the need for rapid results may not be a necessity in some situations, and laboratory-based analysis is a good alternative.

We offer laboratory-based qPCR analysis for detection of single targets as well as metabarcoding analysis for surveying aquatic communities. Our partnership with Precision Biomonitoring allows us to offer our customers high-quality laboratory services from industry leaders at a guaranteed competitive price. Our customers can rest assured that their samples will be analyzed in a top-rated facility by molecular scientists applying the most advanced protocols and methods.

How It Works

Using our eDNA laboratory service is a straightforward process. You collect samples using the ANDe™ eDNA sampling backpack (rentals available) or any alternative eDNA filtration protocol. Once you have collected your samples, send us the individually-labeled filter samples using our eDNA preservation kit, and we will conduct the appropriate molecular analysis of the DNA content from your filters. The results are provided to you in a summarized report. We can archive your samples for up to two years if needed.

Applications

The eDNA laboratory service is designed specifically for detecting aquatic organisms whether they are an invasive, rare, or a species of conservation concern. If you would like to detect a single species that may be difficult to capture with traditional aquatic sampling methods (e.g., netting, electrofishing, etc.), then eDNA + qPCR analysis is a good alternative. Species-specific qPCR analysis provides DNA presence/absence detection along with amount of DNA detected. If you are more interested in detecting several species of fish within a body of water, then metabarcoding analysis is an exciting alternative for biodiversity assessment. With metabarcoding, DNA of several different species of fish can be simultaneously detected which makes it a very powerful molecular tool. To determine what application is best for you, please refer to the scenarios below:

Single Species Detection

(e.g., invasive, rare, conservation species)
applications-single-species

Steps

  1. Water collection and filtration
  2. DNA extraction/purification from filters
  3. DNA amplification by laboratory qPCR
  4. Analysis of qPCR results
  5. Report detailing: species detection (and number of positive replicates) or species not detected

Biodiversity Monitoring

(e.g., taxonomic groups such as "teleost" fish)
applications-single-species

Steps

  1. Water collection and filtration
  2. DNA extraction from filters
  3. DNA amplification by universal primer (PCR)
  4. Next generation sequencing of amplified DNA
  5. Bioinformatics analysis
  6. Report detailing: list of taxa detected and the number of associated DNA sequences

Ready To Get Started?

Contact us today for details and pricing.

labservices@smith-root.com (360) 573-0202 ext. 1930

Laboratories and Protocols

Precision Biomonitoring

Precision Biomonitoring's facilities are accredited by the International Organization for Standardization (ISO/IEC 17025) which is one of the most important certifications for testing laboratories globally. This accreditation indicates that all laboratories and personnel within the facility have demonstrated technical competence and ability to produce precise and accurate test results. Strict and up-to-date protocols are followed to deliver high-quality results. The lab follows a quality assurance and quality control plan to ensure that cross-contamination of samples during analysis is minimized and verified at each step. This type of high-standard laboratory service ensures that our customers can be comfortable and confident with the accuracy of their results.

Reliable results

  • 30 years of delivering accurate and dependable diagnostic and analysis results for clients
  • State-of-the-art technologies and over 100 professionals on staff
  • Internal and external audits of the quality system and test methods done routinely (mature quality assurance system)

Peace of mind

  • ISO/IEC 17025 accredited laboratory
  • Development of new methods to address evolving needs and support research
  • Confidentiality assured

Rapid and routine turnaround times

  • Turnaround times available to suit your needs, ranging from 7 to 21 business days
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