Rapid Environmental DNA Collection and Detection
When combined with the ANDe™ water sampling system, the Biomeme two3™ field-portable qPCR device offers a revolutionary leap forward in the ability of fish biologists to quickly and economically process water samples when surveying for target organisms. No more lab processing delays — get results in as little as 45 minutes.
Living organisms are constantly releasing trace amounts of DNA into their environment via shed cells, gametes, waste products, etc. Once in the environment, environmental DNA (eDNA) can persist for a period of time depending on biotic (e.g., bacteria) and abiotic factors (e.g., temperature and UV exposure). Consequently, free eDNA can be collected from environmental samples such as water, soil, or faeces and analyzed using molecular techniques.
eDNA analysis can be a reliable tool for aquatic species detection even when DNA is present in low concentrations. This makes eDNA especially useful for assessing species distribution or detecting rare species (e.g., threatened, endangered, or invasive) that might be difficult to detect using traditional methods. Similarly, aquatic microorganisms such as bacteria, viruses, fungi, and small parasites can be easily detected using eDNA analysis.
Collection and processing of aquatic eDNA samples for rapid field detection has been simplified with the ANDe™ and Biomeme two3™ systems. Simply collect and filter a water sample with the ANDe™, remove the filter from the single-use filter housing, transfer the filter and extract DNA from it with the Biomeme Field Extraction Kit, detect target DNA with the two3™, and analyze your results from a data portal.