Environmental DNA Field Sampling Techniques

Are you interested in detecting aquatic species from a simple water sample? eDNA species detection is rapidly becoming an industry-standard method, and the number of researchers using eDNA is growing exponentially. Reliable eDNA data depends on good technique and properly trained field crews because there are many instances where contamination/bias can influence species detection results. With the right equipment and good training, eDNA survey methods can save agencies substantial time and money.

Course Objectives

Attendees will receive instruction on the following topics:

• What is eDNA and how do people use it for species detection?
• Proper sampling technique and alternative approaches.
• Basics principles of eDNA detection (PCR, qPCR, Next-Gen Sequencing).
• Discussion of on-site eDNA detection for rapid results, versus laboratory analysis.
• Using eDNA data – "so what do we do with this information?"
• Hands-on field experience using eDNA sampling equipment.

Course Format

Morning

Takes place in our onsite classroom and covers eDNA sampling methodology.

Afternoon

Takes place at a local creek, providing students with hands-on familiarity with equipment operation, and an opportunity to apply the information learned in the classroom. If additional instruction is desired, one-on-one sampling instruction can be scheduled.

Requirements

All attendees should bring a writing utensil, notebook and are suggested to bring chest waders/proper footwear for field use.

Classes Held in Vancouver, Washington

When taught at our headquarters in Vancouver, Washington, we provide lunch, backpack eDNA samplers, eDNA filter packets, and appropriate permits. If you would like to bring your own equipment to use in the class, please feel free to do so. Once course registration is complete, additional details concerning lodging and logistics will be provided.

Classes Held at Customer's Location

If the course is provided at your location, please call about pricing. We require an organizer to provide a classroom with sufficient capacity to seat all participants and an overhead projection screen for a PowerPoint presentation. The class will also need access to a stream and eDNA sampling equipment.

Optional equipment that individuals can bring themselves include a brimmed hat, sunglasses, lifejackets, sunscreen, water bottle, camera, and snacks.